FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n02_f8pax371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n02_f8pax371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28394006
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2412050.849492671094033No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA495420.1744804871845135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC181950.026.18533918
GAGTACG114450.026.16498619
GTACGGG114600.025.70578621
TACGGGG81650.025.22182822
CGCAGAG193550.024.72484215
AGTACGG121700.024.3511320
CAGAGTA216200.022.21263717
AGTGGTA222100.021.9615964
ACGCAGA226200.021.3695714
TCAACGC232900.020.66538411
GCAGAGT232650.020.64371916
AACGCAG251100.019.34615513
GGTATCA252750.019.2605427
TGGTATC254900.019.0679576
GTGGTAT259550.018.8592475
ATCAACG266350.018.21257610
CAACGCA270800.017.97699512
GCAGTGG282600.017.4808982
GTATCAA279650.017.4571888
AGCAGTG286150.017.3858411
Produced by FastQC (version 0.11.2)