FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_islbtn2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_islbtn2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39225499
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG167150.026.81091320
GTACGGG169700.026.28606221
GAGTACG172550.026.0518319
TACGGGG107200.025.7778422
AGAGTAC299600.025.24105818
CGCAGAG315950.024.07681515
AGTGGTA344250.022.3084244
ACGCAGA347950.021.85262314
GTGGTAT362500.021.2328975
GCAGTGG366900.020.8937682
AACGCAG367100.020.80664413
CAGAGTA366950.020.73993517
GGTATCA372450.020.5267147
AGCAGTG383950.019.943691
GCAGAGT383800.019.901316
TGGTATC396450.019.3456
TCAACGC405850.018.87105811
GTATCAA421550.018.2175678
ATCAACG431550.017.76322210
GAGTACT179050.017.59159719
Produced by FastQC (version 0.11.2)