FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_islbtn1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_islbtn1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30496280
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461420.15130369999226137No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355550.11658799040407551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG138450.026.26364320
GTACGGG139450.025.97634721
GAGTACG139850.025.97605519
TACGGGG84850.025.8183922
AGAGTAC256700.024.97049318
CGCAGAG268450.023.7361815
AGTGGTA288850.022.4068764
ACGCAGA290000.022.05560714
GCAGTGG299350.021.4483722
CAGAGTA301400.021.40452617
GTGGTAT301550.021.3831065
AACGCAG301200.021.36147113
AGCAGTG307300.020.7404271
GGTATCA315600.020.7044547
GCAGAGT318500.020.32032616
TGGTATC328400.019.7818986
TCAACGC330400.019.63021711
CGTCTGA102200.019.27491617
GTATCAA350400.018.6183788
ATCAACG350100.018.55519510
Produced by FastQC (version 0.11.2)