FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_f8z2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_f8z2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23993465
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG104400.029.24494721
GAGTACG109750.027.94507819
AGAGTAC197300.026.91036618
AGTACGG116250.026.41223320
CGCAGAG204450.026.10425215
TACGGGG75000.025.71334822
ACGCAGA231200.023.17350614
AGTGGTA232200.023.1488594
CAGAGTA232050.023.11835117
TCAACGC231200.023.09889611
GCAGAGT233750.022.93546116
GGTATCA243550.022.1408947
GTGGTAT243950.022.132885
TGGTATC249250.021.6345636
GCAGTGG255650.021.1878682
AACGCAG253200.021.16000613
AGCAGTG261900.020.7392961
CAACGCA261350.020.65855412
ATCAACG266250.020.21357210
GTATCAA271200.019.9468128
Produced by FastQC (version 0.11.2)