FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_f8z1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_f8z1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34061121
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG113400.023.42529121
GAGTACG116700.022.96981419
AGAGTAC199050.022.21944218
AGTACGG125050.021.27051720
CGCAGAG209500.021.25932915
TACGGGG86050.020.80771822
GAGTACT115800.018.47120
CAGAGTA250050.018.01870717
ACGCAGA251200.017.9087514
AGTGGTA253400.017.8082224
GCAGAGT256650.017.58222416
TCAACGC255900.017.5124211
AACGCAG270300.016.6943313
GGTATCA276550.016.5170977
GTGGTAT286250.015.9332885
TGGTATC286300.015.749756
GCAGTGG301650.015.2801732
ATCAACG298200.015.20180110
CAACGCA296400.015.13116912
TCCGATC119850.014.8823656
Produced by FastQC (version 0.11.2)