FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_f8pax372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_f8pax372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38174134
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG165700.027.6909119
GTACGGG166350.027.58271221
AGAGTAC298900.027.03143318
AGTACGG173750.026.44767620
CGCAGAG314850.025.74970415
TACGGGG114850.025.23227322
AGTGGTA344950.023.7236464
CAGAGTA349050.023.25639317
ACGCAGA359000.022.59260414
TCAACGC363750.022.45881311
GCAGAGT367350.022.1635916
AACGCAG371250.021.93075813
GTGGTAT374400.021.7838445
TGGTATC389400.020.9801526
GGTATCA391750.020.9423667
GCAGTGG399200.020.8024862
ATCAACG401800.020.42643510
CAACGCA407200.020.15555612
AGCAGTG414700.019.8953971
GTATCAA422500.019.4016238
Produced by FastQC (version 0.11.2)