FastQCFastQC Report
Thu 27 Jul 2017
HN7CKAFXX_n01_f8pax371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN7CKAFXX_n01_f8pax371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28394006
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA438960.1545960087491705No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363450.12800236782368787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG111450.026.1257821
GAGTACG111100.026.11492719
AGAGTAC194850.025.90304418
TACGGGG81450.025.62543122
CGCAGAG206000.024.75221615
AGTACGG121000.024.12081120
CAGAGTA235150.021.77187517
ACGCAGA238450.021.32588614
AGTGGTA243700.021.2492854
TCAACGC245250.020.87525611
GCAGAGT245750.020.69240216
AACGCAG259350.019.91326313
TGGTATC262750.019.6692686
GGTATCA264250.019.5445617
GTGGTAT271250.019.1546575
GAGTACT117400.018.42495219
ATCAACG280300.018.38799510
CAACGCA283050.018.28247512
GTATCAA292450.017.6834148
GCAGTGG301500.017.2445222
Produced by FastQC (version 0.11.2)