FastQCFastQC Report
Sun 5 Feb 2023
HN5YJDRX2_n02_br260a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN5YJDRX2_n02_br260a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600917
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC396991.526346284791095No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG380881.4644065919827507No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG37650.1447566377550687No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC28080.10796192266035401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)