FastQCFastQC Report
Sun 5 Feb 2023
HN5YJDRX2_n01_br308a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN5YJDRX2_n01_br308a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833593
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC499261.7619326417026016No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG481621.6996795234883768No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA54440.19212356891056692No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG40610.14331627724941443No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC36280.12803532476258941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)