FastQCFastQC Report
Sun 5 Feb 2023
HN5YJDRX2_n01_br304a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN5YJDRX2_n01_br304a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764321
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG397111.4365553059865335No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC376711.3627577983888268No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG34140.12350229947969141No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC29140.10541467506848878No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA28370.10262918090916358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)