FastQCFastQC Report
Sun 5 Feb 2023
HN5YJDRX2_n01_br280a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN5YJDRX2_n01_br280a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966499
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC331071.6835503094585862No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG302761.5395888835946523No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31430.15982718526681175No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC30330.1542334880414381No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA27140.1380117660878546No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG26510.13480810313150426No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG24970.12697692701598118No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC22320.11350120188212656No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA20160.10251721460321109No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA20070.10205954846658961No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)