FastQCFastQC Report
Thu 8 Jun 2017
HN3YYBGX2_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3YYBGX2_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285507206
Sequences flagged as poor quality0
Sequence length75
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG20394000.068.961338
GTGTCGC38181500.068.888826
TGTCGGT58738700.068.841767
AATTAGA45288150.068.8273548
GTGTCGG59198150.068.813336
TGTCGCT36351250.068.768547
GTCCTCG69750350.068.745096
GTCGGTC51931550.068.740558
TTCGTGT2970300.068.728933
TCCTCGA60700750.068.695967
GAATTAG45469550.068.5896947
GAGCTCT39414900.068.5233612
CAGTCTT51688700.068.495869
GCAGTCT51725450.068.486568
AGGTCTC39991900.068.48513
ACGAGGT44878750.068.4367910
TGAATTA45560950.068.3986746
CCACGAG65946550.068.386988
AGAGCTC30671350.068.37652
TCCACGA74467500.068.3566747
Produced by FastQC (version 0.11.2)