FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28559228
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGT111900.013.66951666
GCGGCCT113700.013.6650912
GCGTTAT115300.013.38738869
ACGCCAG119150.012.9871671
TCCTGGC122300.012.90573228
CGGCCTT121950.012.85537213
TGCGTTA121200.012.76452668
AACGCGG121500.012.730499
CGCGGCC131200.012.24244311
GCTGGCC125850.012.15464240
CCTGGCC135250.011.9029329
TCCTGCG139100.011.24817765
CGCCAGC157200.010.3971342
ACGCGGC150750.010.35314110
CGTTATC152250.010.25314970
TTCCTGG158400.010.2295427
GCAACGC155700.010.2039587
TTTACGG156600.010.19020320
TTACGGT159250.010.042520521
TGCTCAC165800.09.64813450
Produced by FastQC (version 0.11.2)