FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32934538
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGT120450.014.84946866
GCGGCCT127750.013.99739912
GCGTTAT129950.013.92504469
TGCGTTA134300.013.39576268
TCCTGGC136200.013.23326328
GCTGGCC138150.013.17549940
AACGCGG138050.013.0039389
CGGCCTT139400.013.00328313
ACGCCAG141500.012.890521
CGCGGCC148950.012.42805611
CCTGGCC146550.012.32247829
TCCTGCG154350.011.90571465
CGTATGC34900.011.63457444
GTATGCC35750.011.25986445
ACGCGGC166050.011.06395510
TATGCCG34150.010.96735246
CGTTATC169850.010.77731470
TTTACGG178400.010.55338820
TTACGGT176900.010.50446221
CGCCAGC181600.010.5032912
Produced by FastQC (version 0.11.2)