FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38766338
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGT121000.013.68255466
GCGGCCT124400.013.24922712
GCGTTAT128400.013.0845369
TGCGTTA135000.012.52249168
ACGCCAG136100.012.3981871
GCTGGCC136650.012.21864740
TCCTGGC138050.012.19371928
AACGCGG137550.012.059299
CGGCCTT139500.011.99067613
CGCGGCC144350.011.90295211
CCTGGCC146000.011.45792429
GCAAGGG117300.010.9489398
TCCTGCG156700.010.69947365
CACGAGT271550.010.59496268
ACGCGGC173450.09.92632910
CGCCAGC176950.09.8102082
TTTACGG181200.09.77245320
CGTTATC177600.09.69606870
GCAACGC178150.09.6056577
GCCACGA303100.09.59635966
Produced by FastQC (version 0.11.2)