FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42324845
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG534770.12634895650533393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT322850.012.14215468
GCGGCCT105450.011.91356512
CCTGCGT107300.011.710866
GCGTTAT113600.011.27665269
CGCGGCC117200.011.25660911
ACGCCAG116350.010.9828321
ACGAGTT369150.010.74247969
GCAAGGG146050.010.734438
CCTGGCC122500.010.71345529
GCCACGA370700.010.66014866
GCTGGCC121250.010.65283540
TGCGTTA122250.010.62193368
TCCTGGC126950.010.33790228
AACGCGG122650.010.2430189
GCCGGGG42500.010.2145811
CGGCCTT128550.09.99046713
GGCCACG391000.09.90088165
TCCTGCG137500.09.57151265
GAACGGC413450.09.33823761
AACGGCC419500.09.32867962
Produced by FastQC (version 0.11.2)