FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26710661
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG290900.10890782523128124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCGT71800.012.3823266
CACGAGT173750.012.16723468
GCGGCCT71450.012.14611312
CGCGGCC77300.011.40800611
ACGCCAG77750.011.3921051
GCGTTAT75650.011.28907169
GCTGGCC80550.011.08120340
AACGCGG79700.010.976859
CGGCCTT81100.010.91652213
TCCTGGC81900.010.7254828
CCTGGCC83950.010.58871329
TGCGTTA82800.010.56791868
GCCACGA205350.010.482766
GCAAGGG82150.010.3939518
ACGAGTT207800.010.37558269
TCCTGCG92300.09.89773165
GGCCACG220000.09.73700965
GAACGGC224450.09.59112261
AACGGCC233700.09.33134162
CGCCAGC100700.09.2449962
Produced by FastQC (version 0.11.2)