FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14228583
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG160290.11265352284201456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT99150.012.39066268
GCGGCCT33750.011.71653812
CGCGGCC38000.010.86662311
CCTGCGT35250.010.82326366
CCTGGCC38150.010.64152529
GCCACGA116450.010.55014566
ACGAGTT117050.010.5257169
GCGTTAT37700.010.305325569
ACGCCAG39300.010.2447161
GCTGGCC39050.010.21863640
GCAAGGG47350.010.0513828
GTCCGCT7757.2759576E-129.938381
AACGCGG38800.09.9211779
CGGCCTT40600.09.91204813
TGCGTTA41050.09.89065368
TCCTGGC40750.09.87649928
GTCCGGA17100.09.6226871
GGCCACG126750.09.58259365
GCCCCTT15000.09.5694551
GCGGGGG10600.09.2479771
Produced by FastQC (version 0.11.2)