FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22922299
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT54300.011.14926812
ACGCCAG58250.010.4578161
CCTGCGT56300.010.44453466
GTCCGGG19500.010.4131251
GCGTTAT60850.010.41094369
CGCGGCC60250.010.16438411
CACGAGT146500.010.12985368
TGCGTTA62950.010.11930268
GCTGGCC62200.010.07336340
GCCGGGG24400.010.0437511
CCTGGCC64150.09.76538929
TCCTGGC62650.09.71980628
CGGCCTT64350.09.62544913
AACGCGG64250.09.4227529
GTCCGCT14750.09.2567871
GCAAGGG63850.09.09818658
CGGGGGA20150.09.0347471
GCCACGA167650.09.01920866
CGCCAGC76400.08.7497182
GGCCCAT38150.08.5344611
Produced by FastQC (version 0.11.2)