FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25309867
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG326260.12890624830229253No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA255790.10106335209110345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT184600.014.18256968
GCCACGA218900.011.99267166
ACGAGTT227750.011.6491769
GGCCACG227400.011.39053965
GCCGGGG26700.011.1451361
GAACGGC242750.010.71380561
AACGGCC241500.010.69676862
GCGGGGG21900.010.5506081
CATGCGC249250.010.43226328
ACGGCCA249150.010.42464963
GGGGCCC9450.010.37061170
TCATGCG252750.010.32927927
GTGCACA260150.010.19892441
ATGCGCT255900.010.16122429
GGATAAC274200.010.1247291
CCACGAG264550.010.08188267
GGGCTCC258050.010.07869452
GCCCCAT28150.010.0735911
TGCGCTT260950.010.00501130
AGGGCTC263600.09.98602451
Produced by FastQC (version 0.11.2)