FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15680302
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGG211370.13479969964864197No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACA171550.10940478059670025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT117200.013.55864968
GCCGGGG16050.013.305471
ACGAGTT139200.011.56651669
GGGGCCC4853.1941454E-911.54659670
GCCACGA140900.011.32806966
GGGCCCT7101.8189894E-1210.8477521
GGCCACG147400.010.78103365
GCAAGGG52150.010.7367328
CGGGGGA13650.010.0024731
GCGGGGG12950.010.0024731
GAACGGC161100.09.97316561
AACGGCC159950.09.95736462
GTACAGC49250.09.94903333
GCCCCAT15950.09.8770491
ACGGCCA166150.09.79669563
CCACGAG171550.09.38560367
CATGCGC174050.09.229238528
TCATGCG176950.09.15709327
AGGGCGC56600.09.09026570
CAAGGGC62050.09.0800699
Produced by FastQC (version 0.11.2)