FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_lbd38-gr_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_lbd38-gr_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22614188
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCT65000.012.81320712
CCTGCGT64850.012.63007166
GCGTTAT69400.012.40670269
CGCGGCC70950.012.23191611
ACGCCAG70700.011.8341471
AACGCGG72100.011.7944519
GCAAGGG73050.011.7848368
TCCTGGC71550.011.69012228
CCTGGCC73400.011.63392929
TGCGTTA74150.011.5648168
GCTGGCC74600.011.30824940
CGGCCTT75200.011.02863813
CACGAGT144450.010.80689468
CGTATGC27500.010.4376644
TCCTGCG83650.010.21004565
TATGCCG26950.010.13079346
GTCCGCT14700.010.0020831
CGCCAGC89850.09.73809052
GCCACGA162500.09.54223866
GTATGCC29350.09.54098845
Produced by FastQC (version 0.11.2)