FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_bzip1ko1_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_bzip1ko1_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32415772
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1320810.4074590603611107No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1195810.3688975847929829No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT731780.22574813272995628No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA625840.19306651095645663No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG580890.17919980434215788No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA548170.16910595249744476No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG497750.15355179571228475No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC496580.15319086030096707No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC480980.1483783881500647No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA407070.12557775887614214No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA405000.12493918084073394No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC398100.1228105873893733No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC363970.11228176210025169No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC340970.10518645059571619No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG339560.1047514771513077No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT332630.10261362894581069No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG325890.10053439418317724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT187000.028.6056751
CTGACTC225850.023.9106642
CGGCCCG26700.023.72676870
CTAGCAG230300.023.4615278
ACTCTAG246200.022.3753195
GACTCTA255800.021.9871644
TAGCAGA273400.019.980399
TGACTCT287300.019.2717293
CTCTAGC294700.018.8829656
CTATCGA305850.017.608517
TCTATCG317150.016.98111316
GCGCTTC593800.016.59868868
CGCTTCA608300.016.2143169
GTGCGCA265250.015.55749868
GATCTAT349300.015.46809814
GCAGATC355600.015.31216911
ATCTATC353400.015.2986415
AGCAGAT365950.015.0896610
TGCGCAT277200.014.92449269
TCTAGCA576700.014.6969927
Produced by FastQC (version 0.11.2)