FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_bzip1ko1_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_bzip1ko1_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69157534
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC2904090.41992387987691987No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG2668510.38585962304555277No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1543710.22321646113061233No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1441640.20845740393230333No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1321140.19103341654721234No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA1228420.17762634509206185No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC1131300.16358304505189558No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC1116460.1614372195515242No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG988570.1429446573384181No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA871870.12607014009493167No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA834590.12067954881097988No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC811040.11727427990708865No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC775310.11210781460194923No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC755420.10923177220286658No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT742710.10739393917660511No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT733870.10611569811034617No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG709150.10254125024180302No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC704750.10190502165678725No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT698900.10105912683352765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT359550.031.5378631
CGGCCCG60250.025.15382470
CTGACTC459750.024.9918372
CTAGCAG473050.024.4417348
ACTCTAG502950.023.4227375
GACTCTA518850.023.1839284
TGACTCT559250.020.9207173
TAGCAGA575300.020.2435679
CTCTAGC620800.019.1906346
CTATCGA638750.017.99705117
GTGCGCA589500.017.6994468
TCTATCG660500.017.29320316
TGCGCAT611450.017.09259469
GGTGCGC636000.016.47720567
GATCTAT721050.015.93307414
GCGCTTC1307450.015.86418968
CGCTTCA1329600.015.65774169
ATCTATC736850.015.60578615
GCAGATC748000.015.45723611
AGGTGCG682000.015.34546666
Produced by FastQC (version 0.11.2)