FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_bzip1ko1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_bzip1ko1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49224308
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC2071140.42075553403411986No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1875080.3809256191067226No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1080570.21951959182442948No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1057970.21492836425450612No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA858320.17436913485914315No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA843060.17126904049113295No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC773890.157217040003894No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC771670.1567660433133971No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG629770.1279388224208251No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA578830.11759027673888275No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA569100.11561361106386707No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT536750.10904165478568027No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC532720.10822295358626473No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC526830.10702639029481124No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC515040.10463123219528042No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT500380.10165302882470181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT252400.032.18041
CTGACTC323150.025.6468472
CTAGCAG328950.025.3825998
ACTCTAG356950.023.6595675
CGGCCCG36450.023.23791970
GACTCTA369500.023.092734
TAGCAGA396950.021.060839
TGACTCT400450.020.897143
CTCTAGC432450.019.6826746
CTATCGA433400.018.95859117
GTGCGCA409750.018.30574668
TCTATCG447650.018.30027216
TGCGCAT426850.017.6790969
GGTGCGC439050.017.17220567
GCGCTTC915100.016.87146468
GATCTAT493900.016.74234614
CGCTTCA934150.016.53123769
ATCTATC502800.016.39036415
GCAGATC507800.016.34591311
CCGGGGT35200.016.2120421
Produced by FastQC (version 0.11.2)