FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_bzip1ko1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_bzip1ko1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31792676
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1406000.4422402190995184No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1352310.42535268185666414No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT688420.2165341476760245No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG641850.20188611993529582No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA602910.18963801600091795No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA590140.18562136763825732No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC536060.16861116063334838No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC524450.16495937617833742No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG409050.12866170812422334No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA393260.12369515545026787No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA390690.12288679317211297No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC372550.11718107654731548No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC365670.11501705612953121No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT356180.11203209191953517No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT351730.11063239848070669No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC330570.10397677754461435No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC320960.10095406879244767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT169300.030.2325761
CGGCCCG22900.027.5108270
CTGACTC214450.024.2681372
CTAGCAG219500.023.7710788
ACTCTAG227750.023.4197855
GACTCTA234350.022.9095214
TAGCAGA258700.020.304339
TGACTCT260600.020.279513
CTCTAGC286050.018.7568516
CTATCGA292800.017.8676917
GTGCGCA267900.017.32403268
TCTATCG301850.017.18129716
TGCGCAT276400.016.85456369
GCGCTTC600450.016.74699668
CGCTTCA621050.016.23656769
GGTGCGC288400.016.15359567
GATCTAT327850.015.96815214
GCAGATC333000.015.73175511
ATCTATC334650.015.60190415
CCGGGGT23400.015.4101231
Produced by FastQC (version 0.11.2)