FastQCFastQC Report
Wed 9 Mar 2016
HN3L7BGXX_n01_bzip1ko1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3L7BGXX_n01_bzip1ko1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31247841
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1414060.45253046442472616No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1407350.450383116068723No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT744810.23835566751635737No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA654740.20953127609680297No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG618080.1977992655556587No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC589360.1886082305654333No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA543940.1740728263434264No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC543750.1740120221425858No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG453940.14527083647155015No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA419990.13440608584765903No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA418970.13407966329577778No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC388910.12445979867857111No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT372360.11916343276324275No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC367590.1176369273000333No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT361150.11557598491364572No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT356370.1140462792293394No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC346110.1107628523839455No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATG338470.10831788346593291No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC338270.1082538790439954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT180300.031.5914341
CTGACTC218650.026.2188782
CGGCCCG26700.025.2998970
CTAGCAG228150.025.185728
ACTCTAG242700.024.1543525
GACTCTA255000.023.2775944
TGACTCT267600.021.645133
TAGCAGA272500.021.2021989
CTCTAGC303550.019.5891176
CTATCGA307350.018.71852917
TCTATCG317800.017.93761616
GATCTAT342850.016.78004514
GCGCTTC622750.016.71498968
CGCTTCA633450.016.543169
ATCTATC353150.016.28083215
GTGCGCA274600.016.22582268
GCAGATC359750.016.10839311
TCTAGCA591950.015.8967437
TGCGCAT284100.015.67087969
AGCAGAT372900.015.61554210
Produced by FastQC (version 0.11.2)