FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah9251b-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah9251b-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26231656
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3074081.1718970392109442TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG446900.041.80645411
ACACGTC454600.041.12253613
ACGTCTG454200.040.9553315
CACACGT456450.040.9365912
CACGTCT457850.040.65765814
CGTCTGA458150.040.55901316
GATCGGA426850.040.4372631
AGCACAC465100.040.34078610
GAGCACA470250.039.9469
CACAGTC458650.039.8774931
GAACTCC464500.039.82450521
TCCAGTC464450.039.55430225
CAGTCAC465900.039.48351327
AGAGCAC476700.039.4516538
TCGGAAG476600.039.2686163
CCAGTCA468300.039.1966126
GTCACAG468000.039.15585329
CTCGTAT393350.039.0703244
ACTCCAG475000.038.8193523
AGTCACA475700.038.81346528
Produced by FastQC (version 0.11.2)