FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah9251a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah9251a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24286065
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG110000.034.59760311
ACGTCTG114100.033.43144615
ACACGTC116400.032.8465213
CACACGT117450.032.55287612
CGTCTGA117250.032.42071516
CACGTCT117800.032.3441114
GATCGGA111100.031.440721
GTTCCGT119100.030.82780335
CAGTCAC124100.030.59597427
CCGTATC112900.030.51370238
GTCACAG126300.030.0456129
TCCAGTC125800.030.04237225
AGCACAC128400.029.93082410
AGTTCCG124250.029.6916734
GAGCACA130500.029.5335889
CCAGTCA129750.028.97543526
AGAGCAC133700.028.909178
GAACTCC131350.028.85677521
AGTCACA134400.028.41475928
TCGGAAG137050.028.2152483
Produced by FastQC (version 0.11.2)