FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah9120b-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah9120b-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14048529
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC524420.37329175175564644TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC394540.28084079123159444TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG594350.042.92439711
ACACGTC597800.042.72067613
ACGTCTG597050.042.67086815
CACACGT599850.042.5490112
GATCGGA552600.042.460411
CACGTCT600150.042.45076414
AGCACAC602250.042.44910
CGTCTGA601500.042.34786616
GAGCACA606400.042.173469
GAACTCC597500.042.1344821
TCGGAAG606650.042.0837253
CCGATGT586200.042.08355733
TCCAGTC594900.042.03019325
CAGTCAC599250.041.94932627
AGAGCAC610350.041.932968
CCAGTCA599350.041.78082326
ACTCCAG601200.041.7031923
CACCGAT598250.041.67746431
GTCTGAA611450.041.65155817
AGTCACC601150.041.60068528
Produced by FastQC (version 0.11.2)