FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah9120a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah9120a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448425
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1613341.2960193759451497TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG237800.041.95285811
ACACGTC240050.041.55963513
ACGTCTG239800.041.52039715
CACACGT241750.041.3037812
CACGTCT240950.041.2400614
GATCGGA223400.041.1190761
CGTCTGA242100.041.11685616
AGCACAC245550.040.7989710
ACTGACC238200.040.78454632
GAGCACA247050.040.5247049
CGTATGC218150.040.47841344
CAGTCAC244100.040.31231327
TCCAGTC244450.040.1817925
AGAGCAC250000.040.081878
CCAGTCA246700.039.8785426
AGTCACT248200.039.61980428
CTGACCA245550.039.5813533
TCGGAAG252500.039.5767173
ACTCCAG249600.039.5554323
GAACTCC250850.039.4458621
Produced by FastQC (version 0.11.2)