FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah9003mnase3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah9003mnase3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7531513
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCG3501.8189894E-1212.5707757
TGGGCCG7150.012.31220151
AGGGGGG7700.012.29021551
ACCGCGA12150.011.0493141
TAGCCCG4409.094947E-1210.5038461
TAGCCGT7150.010.4653711
TAGGCGG4850.010.4368111
AGGGCGC6600.010.3371191
GGGACAG11300.010.3217472
GGGCGCG2352.8137725E-510.301032
CCGCGAA12400.010.2934772
AGGCGAC8600.010.2363061
AGGCAGC19150.010.2282891
AGGGCCT11300.010.1276031
GGGCCGG2403.547802E-510.0864252
GCGCGCG1750.002814949510.0596253
TGGGACA16950.09.9977621
ACGCGAG4855.456968E-129.9830371
AGGCGGG5101.8189894E-129.9252031
AGGGGGC7450.09.7485361
Produced by FastQC (version 0.11.2)