FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8643top2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8643top2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420547
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT62340.14102327155440267TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16800.031.95034211
CGTCTGA17500.031.30086116
ACACGTC17350.031.19109713
ACGTCTG17400.031.10146515
CACGTCT17500.030.92374214
CACACGT17550.030.83564212
GATCGGA16800.030.5248051
CAGTCAC18550.029.0550827
GAACTCC18700.028.93933321
AGCACAC18950.028.4414610
AGAGCAC19550.028.2437238
GAGCACA19200.028.0711279
CCAGTCA19250.027.76997226
GTCACAT19150.027.68523229
ACTCCAG19950.027.12609123
TCGGAAG20300.027.1029073
TCCAGTC19750.026.95524425
AGTCACA20350.026.91750728
ACATTCC20950.025.51656232
TATCTCG15150.025.12079441
Produced by FastQC (version 0.11.2)