FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8643i3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8643i3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7892636
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT186950.23686636505218284TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT29900.035.0213731
AGTCACG30350.034.71911628
GCACACG32350.034.20424711
CGAGTGG31050.033.65343533
GTCACGA31850.033.01513329
ACGAGTG31950.032.9810732
TCACGAG31750.032.98075530
ACACGTC33350.032.91478713
CACACGT33950.032.7218612
GATCGGA31950.032.0363961
ACGTCTG34900.031.32689315
CACGTCT35300.031.15886714
CGTCTGA36200.030.3843916
AGCACAC36650.030.31124310
CAGTCAC35800.029.9256327
GAGCACA37600.029.6039079
TATCTCG34050.028.814441
GAGTGGA36400.028.76737234
GAACTCC38350.028.16506421
TCCAGTC37700.027.9502725
Produced by FastQC (version 0.11.2)