FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8584d-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8584d-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13936528
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC558130.40047994737283205TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC309740.22225047730682992TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC284600.2042115511123TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG429900.042.81994211
ACACGTC434150.042.4818413
CACACGT435700.042.28526712
ACGTCTG433900.042.26280615
GATCGGA400800.042.218721
CACGTCT435650.042.15363314
GTCACAC431600.042.03530529
CGTCTGA436100.042.03447316
AGCACAC440950.041.9614210
CACACAG435150.041.84924331
GAGCACA443400.041.7497029
GAACTCC438600.041.62980721
CAGTCAC436850.041.61588727
CACAGTG435200.041.500733
AGAGCAC447200.041.454128
TCGGAAG445450.041.317963
TCCAGTC439350.041.26347425
ACTCCAG442600.041.124523
CCAGTCA442300.041.0482426
AGTCACA444900.040.98141528
Produced by FastQC (version 0.11.2)