FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8584c-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8584c-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6599666
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC291720.44202236901079545TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC44450.039.39414229
TCACGCC45100.038.77760330
AGTCACG46100.038.41362828
GCACACG47600.037.6196211
ACGTCTG47900.037.4758615
CGTCTGA48750.036.82243716
ACACGTC48900.036.7544713
CACGCCA48050.036.4884431
CACGTCT49300.036.4562614
CACACGT49550.036.1391312
GATCGGA46200.035.969871
TATCTCG45600.035.16950239
CAGTCAC50250.035.06604827
AGCACAC51550.034.907210
CCAGTCA51700.034.2104826
TCCAGTC51900.033.9514925
GAGCACA53100.033.8468259
CGTATGC43800.033.70145844
CGCCAAT53300.032.7712933
TCGGAAG54750.032.760573
Produced by FastQC (version 0.11.2)