FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8583d-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8583d-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22810423
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC365230.16011539987662657TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC276150.12106307717309758TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG792600.043.0062611
ACACGTC801750.042.63343413
GATCGGA744700.042.469061
ACGTCTG801650.042.46861315
CACGTCT803500.042.44475614
CACACGT804300.042.4353612
AGCACAC808400.042.27165610
CGTCTGA809250.042.0235616
GAGCACA815450.041.9926159
CAGTCAC808300.041.7141627
TCGGAAG819400.041.678953
GAACTCC809200.041.6617821
AGAGCAC823050.041.6556368
TCCAGTC809850.041.50619525
CCAGTCA812100.041.35914626
AGTCACC813600.041.30182328
ACTCCAG815100.041.30093423
CGGAAGA833900.041.0914654
ATCGGAA832600.041.0834122
GTCTGAA830250.041.03217317
Produced by FastQC (version 0.11.2)