FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah8583c-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah8583c-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43559749
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1258530.2889203975899861TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC506780.11634134990079947TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG460050.040.33434711
ACACGTC470200.039.44027713
ACGTCTG468800.039.3984715
CACACGT470450.039.39126212
CACGTCT476000.038.8534514
CGTCTGA475100.038.8204616
GATCGGA436050.038.8041341
AGCACAC485800.038.32320410
GAGCACA493800.037.773839
CAGTCAC485600.037.73250627
TCCAGTC489250.037.347225
CCAGTCA493700.037.07783526
AGTCACC493650.037.02798528
AGAGCAC504600.037.004728
GAACTCC499150.036.91921221
GTCACCT491750.036.7862829
TCGGAAG514000.036.205153
ACTCCAG513250.035.88790523
GTCTGAA516700.035.82697317
CTCCAGT520700.035.20121824
Produced by FastQC (version 0.11.2)