FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah7797mnase3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah7797mnase3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5934186
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCC6350.014.2109761
AGGCGAC7400.012.789411
TAGGCGC4650.012.3064881
AGGGTCT18000.012.2276011
AGGGGCA8400.012.0529211
GGGTCTC11900.011.8364412
GGGACAG8800.011.7544772
AGGGCGC5750.011.4833131
GGGTCGC2402.9540734E-611.0041912
AGGGAAG21650.010.7761031
AGGGGGG6600.010.6713621
AGGCCTG10600.010.5895631
AAGGCGG9550.010.3710551
AGGGCAG14650.010.3663351
AGGGCAA25700.010.36253451
AGGGCGA7800.010.1583151
CCGCGGT4401.0004442E-109.99925923
ACCGCGA9300.09.9398561
AGGGCCA11450.09.8034391
ACCGAGG7200.09.7820811
Produced by FastQC (version 0.11.2)