FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah7797mnase0h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah7797mnase0h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6581736
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAG11850.015.0435782
TGGGACA15850.013.7469671
GGGTCGG3501.6370905E-1111.9473072
TCGGCGG11950.011.78171313
ACCCGGG2251.3591052E-611.7381511
AGGGGGG7350.011.6782631
AGGGCAG15750.011.3189321
AGGGCCC9550.011.2925931
TAGGCGG5300.011.2121491
CGGGGCG2201.3628531E-510.99933216
ACCCGGA7100.010.8495231
TAGGCGC4102.5465852E-1110.7361141
GGCATGG14050.010.4950122
AGGGTCG7850.010.3736851
TAGCCGG4900.010.33077051
GGGCAGG6900.010.20672
TGGCGCG3054.5750494E-710.1025071
AGGGCGT6550.010.0804741
CGGCGGC13800.010.042870514
AGGGGCG6600.010.0041061