FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah5184top2-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah5184top2-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582923
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT264500.47376616156088847TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41350.037.2412411
ACGTCTG41600.036.96455815
CACGTCT41600.036.64726614
CGTCTGA41950.036.39394816
GATCGGA38600.036.1475331
GTCACAC41850.036.11326629
ACACGTC42700.035.9607913
GAACTCC42950.035.5465921
CACACGT43650.035.27893412
CACACTG42600.035.2709131
CAGTCAC43700.035.0878627
CCAGTCA43600.034.86559726
AGAGCAC44150.034.8295678
TCCAGTC43700.034.78581225
GAGCACA44350.034.6229029
TATCTCG41050.034.35156641
GTCTGAA45000.033.92724617
AGCACAC45650.033.92606710
ACTCCAG44950.033.86740523
TCGGAAG45500.033.855133
Produced by FastQC (version 0.11.2)