FastQCFastQC Report
Thu 17 Aug 2017
HN3C3AFXX_n01_ah5184i3h-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN3C3AFXX_n01_ah5184i3h-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7276484
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG16800.031.427633
CGTACGT18400.028.69476334
GCACACG19300.028.26816711
ACGTCTG20550.027.08376515
CGTCTGA20500.026.61326416
CACACGT21100.026.27370612
ACACGTC21000.025.97979413
CACGTCT21450.025.33220314
GTACGTA21700.024.53380435
AGCACAC22900.024.0162310
GATCGGA21750.023.7778761
CAGTCAC23500.022.84137527
GAGCACA24000.022.640819
GTCACCG24300.022.54220229
AGTCACC24650.021.95424528
ACCGTAC24700.021.91025432
TCCAGTC25050.021.34021825
GAACTCC24950.021.2492621
CCAGTCA25300.021.12934726
AGAGCAC26550.021.1291458
Produced by FastQC (version 0.11.2)