FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13079268
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT157540.1204501658655515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG34500.026.9011428
GCCAGTA35150.026.2074721
TATGCCG16700.024.7900546
CGCTATG54000.024.53296929
CGCGTAT19350.024.4274861
CGTATGC16700.024.37688444
GTCGCTA57550.022.90026927
AACGTCG58500.022.64633424
GGCTGCC59600.022.34362443
CCCTGTG60200.022.29285863
GGCGGGC43550.022.10210613
GTGCGAG42150.021.9363710
TGTGTGC45100.021.3434777
CGGGCGC45250.021.11910415
CCGACAT63650.020.9231971
CGGCAAT52600.020.596011
GAACGCT65550.020.5260135
ACATCCG49100.020.37647855
CCAGTTA67600.019.5973455
GCCAGCG51250.019.5216634
Produced by FastQC (version 0.11.2)