FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12873490
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG147850.11484842105753763No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT147130.11428913216229632No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG135920.10558131477944209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA38750.027.7790341
GTGTGCG31700.027.7529018
CGCGTAT18400.025.6883961
CGCTATG54800.022.85292429
GTGCGAG38500.022.67160410
GTCGCTA58100.021.67375227
ACATCCG54200.020.81441355
GGCTGCC60300.020.76849243
TGTGTGC44300.020.7159777
AACGTCG62550.020.62821624
CCCTGTG60450.020.37452963
TGTGCGA43850.020.2203039
CCGACAT64100.020.1840111
TGCGAGT42700.020.0374711
GGCGGGC39150.020.00375213
GCCAGCG58250.019.90027434
CCCTGGT57150.019.8003767
CCAGTAG53200.019.6502742
GTCCTAT58050.019.61225143
CCTATAA59850.019.25298345
Produced by FastQC (version 0.11.2)