FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13209827
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18500.023.12402346
CGTATGC19050.022.818644
CGGGCGC30900.021.54834415
GGCGGGC31250.021.30716313
GCCAGTA33600.020.947661
CGCGTAT13850.020.9254191
GTGTGCG24500.020.418648
CGGCAAT43350.020.2953131
CGCTATG37750.020.10577429
GTATGCC22800.019.0655445
GCGGGCG36100.018.53999314
TAGCCCG36700.018.42482834
GTCGCTA44050.017.3870327
TCGGGCG42200.017.3320527
GGCGCAT39050.017.13966617
ATGCCGT25650.016.67814447
ATCGGGC45700.016.5337966
GTGCGAG30800.016.35386810
CCCTGTG45700.016.30613363
GGCTGCC47050.016.13156343
Produced by FastQC (version 0.11.2)