FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14849297
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19800.021.2572746
GCCAGTA36500.020.9852391
GTGTGCG27800.020.7252228
CGCTATG41000.019.6900329
CGTATGC21650.019.28147944
GTCGCTA45200.018.01313827
CGGGCGC29550.017.74599815
GCCAGCG47150.017.48755534
GTCCTAT48700.017.00181243
GGCGGGC30650.016.99660713
GTGCGAG33550.016.96731210
CCTATAA50150.016.85419545
ATGCCGT27000.016.7386547
GGCTGCC47900.016.70957443
GTATGCC25900.016.38393645
CCGACAT51400.016.3787421
TCCTATA51050.016.1515844
AACGTCG51350.016.12456724
ACATCCG51800.016.0509355
AGGTGCC52700.015.97320630
Produced by FastQC (version 0.11.2)