FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt60240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt60240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12600328
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16750.024.92195546
GCCAGTA34650.023.9972551
GTGTGCG26650.023.8206088
CGTATGC18650.022.5679744
CCAGGCG20450.021.4251433
GTATGCC22300.019.02881845
GTGCGAG34300.018.70842610
GGCGGGC28750.018.59967813
ACGTCTG34800.018.34019915
CGGGCGC29000.018.32037215
GCACACG34950.018.1629911
TCGTATG24150.017.9997643
ATGCCGT24500.017.88336447
CGGGCAT26450.017.7403011
CGCTATG43300.017.68798429
CGGCAAT41400.017.1677991
GTCGCTA45550.017.04155227
TGCGAGT37750.016.90719211
GGCTGCC45250.016.77325643
CAGGCGA26900.016.6726434
Produced by FastQC (version 0.11.2)