FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt60240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt60240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12070993
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG21750.022.3659348
GCCAGTA32800.020.7226261
CGCTATG37250.019.4493829
CGGGCGC25900.019.1811915
GGCGGGC26350.019.11555113
GTCGCTA38900.018.53587327
CGCGTAT13350.018.349721
GGCTGCC40150.018.21642543
TATGCCG17450.018.18884846
CGGCAAT39600.017.6869621
CCGACAT44500.017.5226921
CGTATGC18250.017.3915344
GTGCGAG28650.016.97902510
CCCTGTG42800.016.8467263
GCGGGCG29550.016.8119414
TGCGAGT30000.016.21490111
AACGTCG44200.016.15720724
TGTGCGA30700.015.95762359
TAGCCCG30700.015.9574934
TGTGTGC33900.015.8764697
Produced by FastQC (version 0.11.2)