FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14008590
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19150.026.84295548
CGTATGC19100.026.37134646
GCCAGTA33450.024.8582731
GTATGCC22850.022.64737547
GTGTGCG33400.021.8988278
CGGGCGC42750.020.0139615
GGCGGGC43000.019.81729713
CGCTATG52800.019.667429
TCGTATG27000.019.42194245
CGCGTAT18700.019.1885321
CGGCAAT54400.019.0905151
ACATCCG50300.018.86155755
ATGCCGT26800.018.79450449
GTGCGAG40150.018.30274210
CCTATAA52350.018.18884845
GGCTGCC57150.018.04960843
GCCAGCG51900.018.0141934
GTCGCTA58450.018.00249927
CGTTCAG49750.017.68316357
GCACACG35500.017.59026311
Produced by FastQC (version 0.11.2)