FastQCFastQC Report
Thu 2 Nov 2017
HN37TBGX3_n01_wt6015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHN37TBGX3_n01_wt6015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14242441
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC146680.10298796393118285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA36250.024.3647791
CGCGTAT18700.023.0621011
GTGTGCG29900.022.8473958
CGCTATG51400.021.0756929
TATGCCG14000.020.6996746
GTCGCTA53800.020.1355127
GGCGGGC41800.019.39589913
CGGGCGC41950.019.24423615
ACATCCG54850.019.05803955
CGTATGC15500.018.69647844
CCCTGTG58800.018.54052263
CCTATAA57250.018.50014145
GTCCTAT57700.018.23627943
GGCTGCC59050.018.2283343
GCCAGCG57550.018.22399134
CGGCAAT55300.018.2174761
GTGCGAG37500.018.2160310
AACGTCG58850.018.11472724
CCCTGGT58650.018.05853767
GCACACG32950.018.00913411
Produced by FastQC (version 0.11.2)